In this study, 30 blood samples were collected from Human patients infected with CCHF virus residing in Alrazi Hospital, 30 samples from cattles and 30 from goats from the veterinary medical clinic in Baquba city. Also, 15 blood samples were taken from healthy individuals, 15 samples from healthy cattles and 15 samples from healthy goats as control groups. The study was carried out during the period from, 15th January to 30th June 2023 in Diyala Province. According to the results, the (Mean±S.E) of Human anti IgM antibodies was 2.40±0.26 in patients with CCHF and 2.13±0.23 in cattles with non-significant differences, while the (Mean±S.E) of anti IgM antibodies was 1.72±0.15 in goats with a significant difference between goats and humans (p=0.03). The (Mean±S.E) of Human anti IgG antibodies was 1.42±0.11 in patients with CCHF and 1.27±0.12 in cattles with non-significant differences. Also, the mean ±S.E of anti IgG was 1.24±0.11 in goats with non-significant differences between the animals and Humans. The mean ±S.E of anti-IgM Human antibodies was 2.40±0.26 compared to the control group 0.08±0.05 with a highly significant difference p<0.001. The mean of anti-IgG human antibodies was 1.42±0.11 compared to the control group 0.13±0.06 with a highly significant difference p<0.001. The mean of anti-IgM cattle antibodies was 2.13±0.23 compared to the control group 0.08±0.04, with a highly significant difference p<0.001. The mean of anti-IgG antibodies was 1.27±0.12 compared to the control group 0.09±0.04 with a highly significant difference p<0.001. The mean of anti-IgM goat antibodies was 2.13±0.23 compared to the control group 0.08±0.04 with a highly significant difference p<0.001. finally, the mean of anti-IgG antibodies was 1.27±0.12 compared to the control group 0.09±0.04 with a highly significant difference p<0.001.