This study involved three predominant mango hopper species viz.: Idioscopus clypealis (Lethierry), Idioscopus nitidulus (Walker), and Amritodus atkinsoni (Lethierry). The nymphs and adults were collected from a mango orchard in Bhubaneswar, Odisha, India. To rear the leafhoppers, plastic containers with moist sand and mango twigs as food sources were used in different environments, including room temperature, an open environment, and a B.O.D incubator with controlled temperature (25°C) and relative humidity (75%). The survival and longevity of nymphs and adults were recorded. The results showed that rearing in the B.O.D incubator consistently resulted in the highest longevity rates for both nymphs and adults. Rearing in the open environment had the lowest longevity rates, while room temperature rearing fell in between. These findings highlight the importance of maintaining consistent temperature and humidity conditions for improved longevity in mango leafhopper populations.