Abstract Abelmoschus manihot and Wrightia tinctoria, belonging to the botanical family Malvaceae and Apocynaceae, have been traditionally used by the locals in India for treatment of various ailments. The current study reports the outcome of acute oral toxicity investigation of Abelmoschus manihot and Wrightia tinctoria, on ICR mice. No mortalities or evidence of adverse effects have been observed in ICR mice following acute oral administration at the highest dose of 2500 mg/ kg crude extracts of Abelmoschus manihot and Wrightia tinctoria. This is the first report on the acute oral toxicity of Abelmoschus manihot and Wrightia tinctoria and the findings of this study are in agreement with those of in vitro experiments and thus provide scientific validation on the use of the leaves of Abelmoschus manihot and Wrightia tinctoria.