In this thesis, I develop a new measurement technique, name Hydraulic Force Spectroscopy, to address current limitation in the quantification of mechanical properties of 3D in vitro models. I integrate a traditional testing method of mechanobiology, micropipette aspiration, with principles from optical coherence elastography and civil engineering modeling, to obtain a system capable of measuring spatially resolved mechanical and structural features of 3D models. I validate the technique against standard methods of the field, and show its potential in the context of a spheroid remodeling study and aging, as well as in the case of a drug administration study.