In our study, we aimed to investigate the knowledge, attitude, and behavior of physicians toward hyperbaric oxygen therapy (HBOT). Our study is a cross-sectional study. We included a total of 251 physicians, consisting of Family Medical Specialists, residents of Family Medicine Residency program, contracted residents of Family Medicine Residency program (residents who work actively in primary health units and matched with Family Medicine residency program) and General Practitioners (non-specialized family physicians) working in Ankara, between the dates 01.04.2021 and 26.11.2021. A questionnaire consisting of a total of 51 questions was given to the participants on the internet in order to determine the level of knowledge, attitude and behavior towards HBOT. 251 people, 162 women and 89 men, participated in the study. Of the participants in the study, 137 were residents of the Family Medicine residency program, 57 General practitioners, and 57 Family Medical Specialists. It was observed that 60.2% of the physicians responded with a “no” to the question, “Do you know the indications of HBOT?”. 55% of the physicians did not know about the cost of HBOT therapy. The rate of knowledge of the indication of carbon monoxide poisoning was significantly higher among the physicians who had an HBOT center in their institution (p<0.05). It was observed that 98% of the participants did not know the treatment pressure applied during HBOT, and 97.2% did not know the technique of oxygen administration. While investigating the knowledge, attitudes, and behaviors towards HBOT, we observed that our physicians had some deficiencies, even though they had partial knowledge about it. In our study, we saw that our physicians are aware of their lack of knowledge about HBOT and are open to training on this subject. We think that in order to eliminate this deficiency, a course related to this field can be added to either university curricula or specialist training curricula.