Objective: Some special regions around the world have an important role, in the increasing use of psychoactive substances. The aim of the study is to examine the profile of psychoactive substance users in Lefkosa/Surlarici (walled city) and Gazimagusa/Maras regions, which are considered as special regions in TRNC. Method: The study was carried out in May 2018 with 467 participants between the ages of 18-65. The regions were chosen by randomized sampling method with multiple stratified based on purposive sampling, aiming for maximum diversity. The questionnaires consisted of 30 semi-structured questions, whilst, the use of psychoactive substances were examined with the European Model Questionnaire. Descriptive statistical methods were used and different characteristics were analyzed with chi-square statistical method. Results: The rates of cigarette and Other Psychoactive Drug (OPD) use in the Surlarici and Maras regions were found to be lower than those in Central Region of Gazimagusa and Koskluciftlik regions. The most common substance in the Surlarici and Maras regions is cannabis. Synthetic cannabinoids and sedative/drug. Heroin, ecstasy and LSD substances are used extensively in control centers. Surlarici and Maras regions experience the most immigration from Turkey. The majority of the participants in the Surlarici and the Maras region were not citizens and were mainly illegals. Conclusion: The use of OPD and illegal substances in the Surlarici and Maras regions is higher than the control centers. Socio-demographic characteristics such as low socioeconomic level, low education levels, immigration and unemployment in the Special regions have an effect on substance use. This study shed light on future prevention efforts and found that more attention should be paid to special areas in setting public health policies.