This study was performed to compare between different methods of Saprolegnia treatment hydrogen peroxide H2O2, potassium permanganate KMnO4 and biological treatment non pathogenic bacterial strain Aeromonas hydrophila (NPAH). 120 Oreochromis niloticus were randomly collected and investigated for Saprolegnia fungal infection and infection rate were 10%, 5% and 15% in tolompate village, Elhamool and om-sin village respectively. Obtained Saprolegnia parasitica (S. parasitica) was examined by Polymerase chain reaction (PCR of ITS gene, 750 bp). Results obtained revealed that Kmno4 had the highest effective treatment in S. parasitica infection compared with biological and control treatment. Also, biological treatment with A. hydrophila showed hazourd of bacterial infection. So, KMnO4 recommended in case of S. parasitica infection as treatment of choice followed by H2O2 and a biological treatment with A. hydrophila is not recommended.