Streptomycin resistant isolates of Pseudomonas syringae pv. actinidiae, the causal agent of bacterial canker in kiwifruit, were found in Korea. A total of 734 isolates of P. syringae pv. actinidiae collected between 2008 and 2017 from bacterial canker infections in 111 kiwifruit orchards were assessed for streptomycin resistance. The survival of each isolate was screened against 100 μg/ml of streptomycin. Among 734 isolates, 38 streptomycin resistant P. syringae pv. actinidiae isolates originated from nine orchards were found. Streptomycin resistant isolates belonging to biovar 2 were found in several individual years, but ones belonging to biovar 3 were found in Korea only since 2016. Therefore, to use streptomycin for control of bacterial canker in kiwifruit orchards should be very careful, and it is necessary to check the streptomycin susceptibility of the pathogen before use in kiwifruit orchards.