Mycological characteristics of Phomopsis citri isolates obtained from blighted twigs of citrus cultivation areas in Jeju island were all identified as Phomopsis citri known as the anamorph stage of Diaporthe citri. Pycnidia the isolates sized from 112.2 to 614.4 (av. 328.8) and shaped conical to lenticulata in black. Two types of phialospores, -and -spores were observed from pycnidia. The -spores were hyaline, unicellula, fusiform to ellipsoidal and sized 4.7~8.7 1.9 3.5 (av. 6.7 2.3 ). The -spores were hyaline, unicellula, filiform, curved and often strongly hooked and sized 13.2~27.1 0.8~1.6 (av. 22.1 1.0 ). All isolates grew well and produced abundant pycnidia and spore horns on PDA. In addition, all isolates showed strong pathogenicity to citrus fruit inducing melanose symptoms when artificially inoculated with the pycnidial spores.