Objective: This study aims to examine the prevalence, contributing factors, and healthcare access disparities related to infertility among Indigenous populations in Canada, with a focus on First Nations, Métis, and Inuit communities. Design: A mixed-methods approach combining quantitative analysis of national health survey data with qualitative interviews of Indigenous individuals and healthcare providers was employed to provide a comprehensive understanding of infertility in these populations. Materials and Methods: Quantitative data were sourced from the 2009–2010 Canadian Community Health Survey, analyzing infertility prevalence among women aged 18–44. Qualitative data is collected through semi-structured interviews with Indigenous individuals and healthcare providers in Manitoba and Ontario, focusing on experiences and perceptions of infertility and healthcare access. Statistical evaluations included prevalence estimates and thematic analysis of interview transcripts. Results: Preliminary findings indicate that Indigenous women experience higher rates of infertility compared to non-Indigenous counterparts, with significant barriers to accessing fertility care. Factors such as socioeconomic disparities, geographic isolation, cultural differences, and historical trauma contribute to these disparities. Healthcare providers report challenges in addressing infertility within Indigenous communities due to a lack of culturally appropriate services and mistrust stemming from past experiences with the healthcare system. Conclusions: Infertility among Indigenous populations in Canada is a significant yet underrecognized issue, exacerbated by systemic barriers to healthcare access. Due to a fraught historical past, there has been a paucity of research in this area. Addressing these disparities requires culturally sensitive healthcare policies, increased awareness, and community-based interventions to ensure equitable fertility care for Indigenous peoples.