The surge in the use of botanicals for medicinal purposes cannot be over emphasized. Many stakeholders now perpetrate sharp practices for monetary gains at the expense of clients’ wellbeing. Therefore, proper plant identification must be adhered to and must be followed by authentication in a recognized herbarium. The aim of the present study was to identify the leaf of Peperomia pellucida (L.) Kunth Piperaceae using DNA marker and by employing standard method. A maximum identity of 99% was obtained for P. pellucida (L.) Kunth. This is above the correctness level of 95%.