Clinical mastitis is a common and economically significant disease in dairy animals. A doe with clinical mastitis was brought tothe Veterinary Clinical Complex, College of Veterinary Sciences and Animal Husbandry, CAU (I), Jalukie, Nagaland in 2022.The milksample was collected from the affected teat. Then, the milk sample was cultured, and performed bacterial isolation test as well as an antibiotic susceptibility test (ABST) to check antimicrobial susceptibility. The growth of smooth, mucoid, and circular colonies was found on nutrient agar media and the colonies appeared as gram-positive cocci with gram staining technique. Furthermore, the bacterial isolates were sensitive to Gentamicin, Cefoperazone, and Ceftriaxone.The findings of this study have provided valuable insights for selecting appropriate antibiotic therapy for the effective therapeutic management of mastitis in goats. Highlights 1. Staphylococcus spp. were isolated from the milk sample of a doe suffering from clinical mastitis. 2. Selection of appropriate antibiotics based on antibiotic susceptibility test (ABST), thus combating potential emergence of resistant bacteria. 3. Optimal supportive care and effective therapeutic management of clinical mastitis in goat.