Purpose: Purpose of this study to evaluate the effect of implant position on prosthesis movement of mandibular two implant-supported overdenture under simulating biting force. Materials and methodology: 30 experimental overdentures (N=30) were made for an edentulous mandibular test model, which included artificial mucosa. Based on implant position, 30 samples overdentures were divided into three groups (n=10) (i.e., A and E, B and D,in between B and D). For this study, the ball and O-ring attachment system was used. 100 N vertical loads were used on the left first molar region, 55N on the left canine, and 40N on the mid-anterior area. The loading points vertical displacement as well as the right distal edges horizontal and vertical displacements were measured.A 1-way analysis of variance test was used to statistically analyse the displacement values with the implant position as a factor, (p=.05) was used to statistically analyze the values of the vertical and horizontal displacement at the distal edge of the overdenture. Results:The mean horizontal displacement at right distal edge on incisor, canine and molar loading was highest in Group III with p value < 0.05. when compared in between groups with paired t tests there was no statistically significant difference on incisor loading between group II and Group III with p value >0.05. Mean vertical displacement at right distal edge on incisor loading was highest in group II and on canine and molar loading, it was highest in Group III. Conclusion:Change in implant position influences the displacement of implant supported overdenture.