Background:-Pseudomonas species are Gram negative bacteria which are resistant to commonly used antibiotics. Due to the formation of biofilm antimicrobial therapy is reduced & leads to chronic bacterial infections. Therefore, we need to isolate species of Pseudomonas from different clinical samples, detect ESBL & biofilm production in them. Objective:-1. To determine percentage of Pseudomonas species isolated from different clinical samples. 2. To determine the antibiogram of Pseudomonas species isolated from the samples. 3. To detect ESBL & biofilm production in Pseudomonas species. 4. To correlate biofilm & ESBL production. Method:-The susceptibility of different clinical samples of Pseudomonas spp.was determined by using antibiotics on Muller Hinton Agar by the Kirby -Bauer disk diffusion method using Clinical & Laboratory Standards Institute (CLSI) standards. Confirmatory test for ESBL production was done as per Clinical and Laboratory Standards Institute (CLSI) 2021guidelines. For the detection of biofilm Tissue Culture Plate (TCP) method was used. TCP is considered as the gold-standard method for biofilm detection. Result:-Out of total 171 Pseudomonas aeruginosa samples 30 were ESBL producers (17.5%) & 141 were Non ESBL Producers (82.5%), 12.8% were ESBL Producers & Strong Biofilm producers, 82.45% were Strong biofilm producers & Non ESBL producers. Conclusion:-Phenotypic detection of ESBL & antibiotic resistance in P.aeruginosa should be done & both the results should be taken in consideration for better representation of resistant strains, this will help clinicians to give appropriate antibiotics so that they can treat the infections timely.