Introduction: Urinary tract infection (UTI) is one of the most common clinical entities encountered by physicians globally. Most of the uropathogens exhibit Multiple Drug Resistance(MDR) mechanisms especially in the members of Enterobacteriaceae. As there is increase in resistance rates, there is necessity in opting alternative treatment Strategies in cases of UTI like Fosfomycin which seems to be a good option attracting the clinicians interest globally. Objective: To evaluate the Fosfomycin susceptibility in bacterial isolates from uropathogens. Methodology: A total of 624 urine samples were included in the study, out of which 84 isolates satisfying Kass concept were processed and identified by standard protocol and all the isolates were tested for Fosfomycin susceptibility by using Kirby Bauer disc diffusion and E-test, interpreted as per Clinical Laboratory Standards Institute(CLSI) guidelines. Results:Among the isolates majority were Escherichia coli(60.71%) followed by Klebsiella species(26.19%), Enterococcus species(8.33%), Staphylococcus saprophyticus(2.38%) and Proteus species(2.38%) out of which 54.76% were Extended-spectrum-beta-lactamase-producing(ESBL) organisms, 40.47% were Multi Drug Resistant Enterobacteriaceae(MDRE) and 15.47% were Carbapenem Resistant Organisms(CRO). Fosfomycin susceptibility was observed highest in Staphylococcus saprophyticus(100%), Proteus species(100%) followed by E.coli(96.7%) Klebsiella species(95.45%) and Enterococcus species(85.71%). Conclusion: Our study mainly focussed on Fosfomycin susceptibility in various uropathogens where Fosfomycin was found to have a promising role in treating UTI with both Gram positive and Gram negative organisms and can be considered as a drug of choice for empirical oral treatment in cases of UTI.