Background:Breast cancer is most common cancers in reproductive women and also the leading cause of cancer deaths worldwide. First line of invasive investigation of breast lump is Fine needle aspiration cytology (FNC) to categorized the lesion as benign or malignant. Recently, an expert panel published [International Academy of Cytology](IAC) Yokohama System for reporting and classification of breast lump cytopathology. The aim of the present study was to evaluate the applicability of Yokohama system of classification and reporting. Materials and Methods: In this retrospective studya total of 612 breast FNC cytology specimens from 1st January 2021 to 31st October 2021 were obtained. These were reclassified according to Yokohama system of reporting. The Risk of malignancy (ROM), sensitivity, specificity, Positive Predictive Value (PPV), Negative Predictive Value (NPV) and diagnostic accuracy of breast FNC were calculated. Result: Out of 612 cases histo-pathological correlation were available in 355 cases. ROM is calculated for each category of Yokohama system. ROM for each category was 10% for category 1, 2.5% for category 2, 44.4% for category 3, 100% for category 4 and 5. Sensitivity, specificity, positive predictive value, negative predictive value and diagnostic accuracy of FNAC were respectively 94.3%, 96.2%, 89.1%, 98.4% and 99.1% , respectively. Conclusion: In conclusion, the IAC Yokohama System for reporting Breast cytopathology is simple but it simultaneously enables greater diagnostic accuracy, consequently leading to better patient care.