This topic explains to us the technique of drug discovery. In this we take a protein sample which under-go sequencing forcers by using either with chemical and chromatography process or by mass spectroscopy which gives us the detailed information of monomeric amino acid groping or sequencing. After this the protein sample undergoes crystallization for the X-ray crystallography this helps to know about the 3D structure of the protein. By getting the information of protein we can now perform the high throughput screening which is an interrogation of more than 10,000 compounds that have a drug life property. High throughput screening is a fully automatic process in which with the help of advanced technology and robotics helps to test many compounds from compounds library at the same time. In this, we learn about protein sequencing, mass spectroscopy, HPLC, TLC, X-Ray crystallography, high throughput screening (HTS), and the phenomena of drug discovery.