Aim: To evaluate the efficacy and tolerance of triple therapy in eradication of Helicobacterpylori. Subjects and methods:Our study is a prospective observational study conducted at the gastroenterology dept in tertiary care hospital, Hyderabad, and accepted by the institutional ethical committee. A total of hundred subjects who were positive for infection of helicobacter pylori was screened by the procedure of endoscopy RUT mentioned in the study based on inclusion&exclusion criteria. Subjects were treated with triple therapy for fourteen days. After completion of fourteen-days therapy, assessment of Helicobacter pylori eradication was carried out after four weeks by the stool antigen test. Results:In the total of hundred subjects, 60 male and 40 female subjects and 44.66 yrs is the mean value of age. total hundred subjects, successful eradication rate was seen in 91 subjects and eradication failure was seen in 9 subjects against helicobacter pylori infection. Conclusion:Standard triple therapy is considered as first-line therapy for the infection of Helicobacter pylori eradication for the past two decades and the effectiveness of triple therapy is variable regionally. so, we are concluding that a 91% success rate was seen in our region with triple therapy and it is well tolerated because no subjects were discontinued therapy due to adverse effects.