The study sought to determine the effect of bosuball training on VO2 max in college football players. It was hypothesised that college football players' VO2 max would differ significantly as a result of bosuball training. For the current study, 30 male college football players from Malappuram district, Kerala state, India, were chosen at random, with ages ranging from 18 to 25 years. For the current study, a pre-test-post-test random group design with control and experimental groups was used. Group 'A' underwent bosuball training, while Group 'B' did not. The beep test was used to assess VO2 max. The data were gathered before and after twelve weeks of training. The data were analysed using Analysis of Covariance (ANCOVA). The level of significance was set to 0.05. The bosuball training had positive impact on VO2 max and agility among college football players.