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Preprint . 2025
License: CC BY NC SA
Data sources: ZENODO
ZENODO
Preprint . 2025
License: CC BY NC SA
Data sources: Datacite
ZENODO
Preprint . 2025
License: CC BY NC SA
Data sources: Datacite
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Statistical Evaluation of a Ribosome-Only Protocell in Prebiotic Environments: Implications for the RNA World Hypothesis

Authors: Reza Hashemi;

Statistical Evaluation of a Ribosome-Only Protocell in Prebiotic Environments: Implications for the RNA World Hypothesis

Abstract

The RNA World hypothesis posits that RNA-based systems, such as ribosomes, could sustain early life without additional molecular support. We test this by simulating a protocell composed solely of a modern ribosome (large and small subunits, ~4,500 nt rRNA, excluding mRNA, tRNA, proteins, or other elements) in hydrothermal vents (80 ± 15°C, pH 6.0 ± 0.5, oxidative stress 0.45 ± 0.18 mM), ice (-5 ± 3°C, pH 7.0 ± 0.4, oxidative stress 0.08 ± 0.04 mM), and wet-dry cycles (25 ± 10°C, pH 6.8 ± 0.6, oxidative stress 0.35 ± 0.15 mM). An Agent-Based Model (ABM) simulates 1,000 protocells over 100,000 steps (~100 division cycles) with 1,500 replicates. Without supporting elements, ribosomes exhibit negligible function: median survival of 20,000–40,000 steps, proto-translation rates of 0.001–0.003 mM/hr, rRNA mutation rates of 5.5–6.0 × 10⁻⁷, and lineage divergence of 20–30%. Statistical analyses (ANOVA, regression, Kaplan-Meier, bootstrap) confirm robust failure (p 0.85, 95% CI ±1.0–2.0%), with ice marginally better but insufficient. Compared to supported systems (970,000 steps, 0.068 mM/hr), ribosome-only protocells are 96–98% less viable. These simulations statistically refute the RNA World hypothesis, supporting the Matter World Hypothesis (MWH) that molecular synergy is essential. Results are computational and require experimental validation.

Keywords

Ribosome-Only Protocell, RNA World Hypothesis, Agent-Based Modeling, Matter World Hypothesis, Hydrothermal Vents, Ice, Wet-Dry Cycles, Statistical Refutation

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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