3. Result In dechlorinated water containing rabbit food at a concentration of 0.16 g /L, both the BORA laboratory strain and the natural strain of Ae. aegypti larvae exhibited high survival rates, with the natural strain having a slightly higher rate (94.00%) compared to the laboratory strain (92.42%). Both ovitrap prototypes demonstrated high efficacy in laboratory experiments, achieving a trapping success rate between 96.74% and 100% (Table 1). Only a minimal number of adult Ae. aegypti mosquitoes managed to escape the traps. Four adult Ae. aegypti mosquitoes of the BORA strain out of 558 escaped the F14 ovitrap, resulting in a 99.28% trapping efficacy. Similarly, the F9 ovitrap achieved a 99.27% efficacy, with only 4 out of 551 BORA strain adults escaping. The standard black trap (SO) collected the most eggs (average 287.40), followed by the novel ovitrap (ALO) with 253.40, and the transparent bucket trap (LVT) with 131.60. While SO and ALO showed no significant diference, dark containers (SO and ALO) attracted significantly more eggs than the bright LVT. However, SO requires periodic larval removal to prevent adult emergence. The ALO’s egg attraction, comparable to SO, combined with its ability to retain emerged adults, suggests superior mosquito control efectiveness compared to both LVT and SO. Field evaluation in Ban Na Chum Kham, Ubon Ratchathani Province, demonstrated the efficacy of ovitraps against Ae. aegypti. Strategic deployment of F14 and F9 ovitraps across 50 households yielded 4459 larvae (average 89/trap). The F14 ovitrap consistently captured significantly more larvae (70.60%, 3148) than F9 (29.40%, 1311). Significant diferences in larval capture between F14 and F9 were observed in weeks 1, 3, 7, and nine (p <0.05). F14 showed a significant decrease in larval capture from Week 1 to 11 (p = 0.018). Statistical analysis confirmed significant diferences in capture rates between F14 and F9 over time (Mauchly’s W = 0.349, p <0.05; Greenhouse–Geisser = 2.316; Wilks’ lambda = 0.807). These results indicate the F14’s superior initial capture rate, followed by a decline, while F9 maintained a consistent rate (Table 2, Figures 1, 4, 5 (c)). Bimonthly larval collections showed a consistent decline, indicating reduced Ae.aegypti oviposition, attributed to F14 and F9 ovitrap deployment. Larval counts decreased from 829 to 649 weekly (Figures 5 and 6), with all 50 households initially showing larvae presence. The F14 ovitrap captured significantly more larvae in weeks 5 and 7, highlighting its ongoing efficacy. Linear regression confirmed this downward trend (y � 851.49 − 2.719 x, R 2 � 0.833, p � 0.011) (Figure 5 (b)). Interestingly, a significant negative correlation was found between rainfall and larval counts (B � −2.036, SE � 0.403, β � −0.930, t � −5.049, p � 0.007) (Figures 5 (c) and 5(d)).

Published as part of Haruay, Surat, Piratae, Supawadee, Niamhom, Kanatid, Loyha, Kulchaya, Srisura, Denduangdee, Yaoup, Kongkaew, Pimpabud, Suwat, Netthip, Worasorn & Khampoosa, Panita, 2025, Efficient All-Life-Cycle Ovitrap for Effective Aedes (Stegomyia) aegypti (Linnaeus, 1762) (Diptera: Culicidae) Control With Low Operational Costs, pp. 1-15 in Psyche: A Journal of Entomology (5543382) (5543382) 2025 (1) on page 5, DOI: 10.1155/psyc/5543382, http://zenodo.org/record/15391623