Tissue culture has emerged as a transformative technology in vegetable crop production, offering rapid propagation of high-quality plants, disease-free stock, and genetic improvements. This article explores various tissue culture techniques, including micropropagation, callus culture, organogenesis, somatic embryogenesis, and anther/pollen culture, highlighting their applications in commercial vegetable farming. The benefits of tissue culture, such as enhanced productivity, conservation of rare germplasm, and year-round cultivation, are discussed alongside challenges like high costs and skilled labour requirements. Strategies for cost reduction, including alternative culture media and natural gelling agents, are also reviewed. With continuous advancements, tissue culture holds immense potential for sustainable agriculture and global food security.