
doi: 10.5274/jsbr.26.3.40
Ten fluorochromes and seven stains were evaluated as indicators of viability of sugarbeet (Beta vulgaris) pollen. The reactions of these materials were com pared with in vitro germination of pollen in a liquid medium. Among these 17 materials, fluorescein diacetate (FDA), 3-(4,S-Dimethylthiazol-2-yl)-2,S diphenyl-2H-tetrazolium bromide (MTT), and isatin exhibited viability reactions that were positively re lated to in vitro pollen germination. However, only the FDA fluorescence reaction was related consis tently to germination in all pollen sources. The linear regression model of germination (Y) and FDA reac tion (X) was Y = O.6X 12. The fluorochromes 4',6 diamidino-2-phenylindole (DAPI), ethidium bro mide, and aniline blue each exhibited some potential to detect and display the presence of DNA, sperm cells, and in vivo pollen tubes, respectively. Additional
| selected citations These citations are derived from selected sources. This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically). | 1 | |
| popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network. | Average | |
| influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically). | Average | |
| impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network. | Average |
