Brine Shrimp Lethality Test Ekstrak Etanol Herba Pugun Tanoh (Picria fel terrae L.)
Copyright policy )Brine Shrimp Lethality Test Ekstrak Etanol Herba Pugun Tanoh (Picria fel terrae L.)
Herba Pugun Tanoh (Picria fel terrae L.), spesies tumbuhan dari famili Scrophulariceae. Penelitian ditujukan untuk penentuan persen kematian dan nilai LC50 dari ekstrak etanol herba Pugun Tanoh pada larva Artemia Salina L. secara Brine Shirmp Lethality Test (BSLT). Konsentrasi perlakuan meliputi 180,0 ppm; 200,0 ppm; 220,0 ppm; 240,0 ppm; 260,0 ppm; 280,0 ppm; serta 300,0 ppm serta 0,0 ppm sebagai kontrol negatif dengan perlakuan tiga kali ulangan. Setiap perlakuan digunakan larva berumur 48 jam sebanyak 10 ekor. Toksisitas diidentifikasi berdasar persen kematian larva dengan analisis probit (LC50). Hasil diperoleh persamaan linear, Y = 11,222X+65,427. Nilai LC50 tercapai saat konsentrasi 188,691 ppm. Toksisitas ekstrak etanol herba Pugun Tanoh diuji secara BSLT menunjukkan sifat toksik, ada potensi sebagai antikanker karena LC50 < 1000,0 ppm
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