Produção e Caracterização de Levanasacarase de Bacillus subtilis natto

descriptionPublicationkeyboard_double_arrow_right Article , Other literature type 01 Nov 2015Publisher:Editora Edgard BlücherJournal:Anais do V Simpósio de Bioquímica e Biotecnologia

Authors: Gabrielly Terassi Bersaneti; Janaina Mantovan; Dionísio Borsato; Maria Antonia P. Colabone Celligoi;

doi: 10.5151/biochem-vsimbbtec-21970

Produção e Caracterização de Levanasacarase de Bacillus subtilis natto

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Abstract

RESUMO O objetivo deste trabalho foi produzir levanasacarase de Bacillus subtilis natto e avaliar os parâmetros cineticos pH e temperatura e o efeito da concentracao do substrato sacarose na atividade enzimatica. A atividade de hidrolise da levanasacarase no extrato bruto obtido do cultivo de B. subtillis natto, apos 24 h, 150 rpm e 37°C foi de 18,7 U/mL. A caracterizacao parcial demonstrou que as condicoes otimas de reacao da enzima foram pH 6,0 e 50°C. O Km para sacarose foi de 20mM.

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Londrina State University
Brazil

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