ABSTRACT Trauma to the tooth mainly results in pulpal injury, and these injuries during formative stage can result in incomplete root formation. This can result in failure in closure of root apex, resulting in wide open apex. This causes problem for the conventional root canal therapy as there is no apical stop against which the obturation material can be condensed. The management of a nonvital tooth with open apex is aimed at creation of an apical barrier. The newly introduced bioactive dentin substitute commercially available as biodentin is a promising material. It is a cement for stimulating hard tissue formation, i.e., the formation of reactive or reparative (tertiary) dentin. This case report describes the management of a traumatized permanent maxillary central incisor with an open apex. How to cite this article Nazar F, Nair KR, Praveena G, Anilkumar A, Rajendran R. Single-visit Apexification using Biodentin. Cons Dent Endod J 2017;2(1):40-42.