On January 22, 2024 the Helmholtz Open Science Office hosted the Second Helmholtz Open Science Forum on the topic of Open Science and Transfer. The online event addressed various aspects and issues around the interplay of Open Science, Technology Transfer, Knowledge Transfer and Citizen Science at the Helmholtz Association. Together with the participants important overlaps were identified against the backdrop of the digital transformation. Open science as a standard for scientific work creates foundations for successful transfer - and both topics can complement each other very well. The Helmholtz-internal event gave insights into current projects and initiatives relating to transfer to society, business and industry. Moreover, Helmholtz initiatives for Citizen Science and the successful practical implementation of Open Hardware were presented. The event also offered opportunities for networking and the exchange of ideas. This report documents the Second Helmholtz Open Science Forum on Open Science and Transfer.