Objective. To adapt and validate the generic TFA acceptability questionnaire to Spanish. Methods. In the initial phase of this two-phase observational study, a translation and adaptation of the original instrument was performed. The second phase consisted of applying this version to 194 older adults after a pilot hearing screening and a standard preventive medical examination. Construct validity was assessed by Exploratory Factor Analysis, and internal consistency with McDonald's omega (ω). Results. Both procedures yielded the same two-factor solution. The first factor comprised items related to “affective attitude”, “perceived effectiveness”, “intervention coherence”, and “self-efficacy”; the second factor included items on “ethicality” and “opportunity costs”. The item 'burden' was removed due to its lack of significant factor loadings. The first factor accounted for 18.96% of the variance of the hearing screening and 26.28% of the preventive medical examination; the second accounted for 14.97% and 20.44%, respectively. Regarding reliability, the instrument demonstrated an ω = 0.61 for the hearing screening and ω = 0.73 for the preventive medical examination. Conclusion. The final instrument comprises six items evaluating specific acceptability constructs and one item addressing overall acceptability. It features a two-factor underlying structure, demonstrating high external reliability and acceptable internal consistency.