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FIRST REPORT OF PERSIMMON CRYPTIC VIRUS AND PERSIMMON VIRUS A IN KOREA

Authors: Cho, I.S.; Park, M.J.; Kwon, S.J.; Choi, G.S.; Hammond, J.; Lim, H.S.;

FIRST REPORT OF PERSIMMON CRYPTIC VIRUS AND PERSIMMON VIRUS A IN KOREA

Abstract

In 2014, a total of 77 persimmon (Diospyros kaki Thunb.) trees from Korean commercial orchards were surveyed for Persimmon cryptic virus (PeCV) and Persimmon virus A (PeVA). Leaf samples were collected from symptomatic trees with necrosis (two), or mosaic and leaf malformations (one) and 74 asymptomatic trees. Total RNAs were extracted using the NucliSENS easyMAG system (bioMérieux) and subjected to RT-PCR using specific primer pairs PeCV F/PeCV R (5’-TTCCAATGGCAGACCAAGG-3’/5’-TGTGTAGGTCGGATGACG-3’; design based on GenBank accession number HE805114) and PeVAfor/PeVArev (Morelli et al., 2014). PCR products of the expected sizes (526 bp and 250 bp for PeCV and PeVA, respectively) were directly sequenced. Results of RT-PCR revealed 67 PeCV (87%) and 11 PeVA (14.3%) positive samples, including nine samples with mixed infections of PeCV and PeVA. BLASTn of consensus sequences revealed 99% nucleotide sequence identity to Italian PeCV isolate SSPI (HE805114) and 98% to Japanese PeVA isolate (AB735628) respectively. The consensus sequences were deposited in GenBank as AB968365 (PeCV) and LC177111 (PeVA). Mixed infections of both viruses were detected in one plant showing vein necrosis, and in eight asymptomatic samples. To our knowledge, this is the first report of PeCV and PeCV infection of persimmon in Korea.

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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Average
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