A leaf disc regeneration system was developed for passion fruit, Passiflora edulis Sims. Leaf discs were cultured on a modified MS medium containing 8.90 µM BAP or on 8.90 µM BAP and 2.32 µM KIN. Shoots appeared within four weeks. These could be rooted when transferred to the same medium containing 0.54, 2.69 and 5.37 µM NAA. Leaf discs cultured on medium supplemented with 11.42 µM IAA, 10.74 µM NAA and 1.86 µM KIN formed callus only. Those cultured on medium supplemented with 10.74 µM NAA and 2.32 µM KIN formed callus and roots.Keywords: Leaf disc regeneration, passion fruit, Passiflora edulis, tissue culture