Rapamycin regulates biochemical metabolites
Copyright policy )Rapamycin regulates biochemical metabolites
The mammalian target of rapamycin (mTOR) kinase is a master regulator of protein synthesis that couples nutrient sensing to cell growth, and deregulation of this pathway is associated with tumorigenesis. p53, and its less investigated family member p73, have been shown to interact closely with mTOR pathways through the transcriptional regulation of different target genes. To investigate the metabolic changes that occur upon inhibition of the mTOR pathway and the role of p73 in this response primary mouse embryonic fibroblast from control and TAp73(-/-) were treated with the macrocyclic lactone rapamycin. Extensive gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry (LC/MS/MS) analysis were used to obtain a rapamycin-dependent global metabolome profile from control or TAp73(-/-) cells. In total 289 metabolites involved in selective pathways were identified; 39 biochemical metabolites were found to be significantly altered, many of which are known to be associated with the cellular stress response.
- University of Insubria Italy
- University of Calabria Italy
- University of Leicester United Kingdom
- Medical Research Council United Kingdom
- University of Rome Tor Vergata Italy
autophagy, 570, Sirolimus; cell death; Animals; Glutathione; metabolism; mTOR; Mice; Methionine; Mice, Knockout; p53 family; Metabolome; Nuclear Proteins; MEF; p73; Biosynthetic Pathways; Cells, Cultured; Pentose Phosphate Pathway; autophagy; rapamycin; Glycolysis, Cells, Knockout, p73, Pentose Phosphate Pathway, Mice, Methionine, Report, Animals, Sirolimu, Settore BIO/10 - BIOCHIMICA, Cells, Cultured, Nuclear Protein, Mice, Knockout, Sirolimus, MEF, Cultured, Animal, rapamycin, Nuclear Proteins, Biosynthetic Pathway, Glutathione, Biosynthetic Pathways, cell death, mTOR, Metabolome, p53 family, metabolism, Glycolysis
autophagy, 570, Sirolimus; cell death; Animals; Glutathione; metabolism; mTOR; Mice; Methionine; Mice, Knockout; p53 family; Metabolome; Nuclear Proteins; MEF; p73; Biosynthetic Pathways; Cells, Cultured; Pentose Phosphate Pathway; autophagy; rapamycin; Glycolysis, Cells, Knockout, p73, Pentose Phosphate Pathway, Mice, Methionine, Report, Animals, Sirolimu, Settore BIO/10 - BIOCHIMICA, Cells, Cultured, Nuclear Protein, Mice, Knockout, Sirolimus, MEF, Cultured, Animal, rapamycin, Nuclear Proteins, Biosynthetic Pathway, Glutathione, Biosynthetic Pathways, cell death, mTOR, Metabolome, p53 family, metabolism, Glycolysis
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