Chromosomal mapping of the GFP-LC3 transgene in GFP-LC3 mice
Copyright policy )Chromosomal mapping of the GFP-LC3 transgene in GFP-LC3 mice
GFP-LC3 mice are widely used to monitor autophagy. Here, we report chromosomal mapping of the GFP.LC3 transgene in this mouse model. The transgene was located in a very distal region of chromosome 2, immediately upstream of a putative open reading frame that is thought to be a part of a pseudogene. With this information, we were able to generate a PCR system to easily determine zygosity. Homozygous GFP-LC3 mice (GFP-LC3(tg/tg)) were healthy, fertile and did not show an abnormal phenotype. Thus, GFP-LC3 integration in this model appears not to affect functions of other genes, justifying use of this mouse model.
Male, Recombinant Fusion Proteins, Green Fluorescent Proteins, Chromosome Mapping, Mice, Transgenic, Mice, Phenotype, Autophagy, Animals, Female, Transgenes, Microtubule-Associated Proteins
Male, Recombinant Fusion Proteins, Green Fluorescent Proteins, Chromosome Mapping, Mice, Transgenic, Mice, Phenotype, Autophagy, Animals, Female, Transgenes, Microtubule-Associated Proteins
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