Background and Aim: Cystic artery (CA) is one of the important structures to be ligated during cholecystectomy. Recurring complications such as hemorrhage or liver injury has attracted surgeons, radiologists, and anatomists to do research on the topic. Materials and Methods: Thirty-two formalized cadavers were used for this study. After opening the abdomen, lesser omentum was separated, followed by fine dissection of CA. Findings were recorded and variations were photographed. The collected data were analyzed, and the prevalence was expressed as percentage. Results: In 68.75%, single CA was passing through the Calot's triangle (CT), and in 4.5%, double CA was passing through the CT. Conclusion: Accurate knowledge of CA anatomy is essential to avoid iatrogenic extrahepatic biliary injuries in surgeries related to this region. We have focused to explore CA variations to help the surgeons and radiologists.