ABSTRACT Background: Immersion media such as ophthalmic irrigation solution, eye lubricant solution, and neutral soap solution will be the best, which will help in the longevity as well as the color stability of ocular prostheses. Aim: The aim of this study was to evaluate and compare color stability of ocular prosthesis after immersion in three different immersion media. Materials and Methods: A total of 90 samples of scleral acrylic resin were prepared, which were painted with natural dry earth pigment NEAS and then were divided into three groups. These samples were checked before and after immersion in respective media after 8 weeks. To assess the color change, a spectrophotometry test was performed and the results were statistically analyzed by one-way analysis of variance and post hoc Tukey’s test. Results: Among all the three groups, the samples immersed in neutral soap solution proved to be most color stable, followed by eye lubricant solution, and the least color stable among the three was ophthalmic irrigating solution. Conclusion: The neutral soap solution proved to be the most color stable.