ABSTRACT Purpose: The extraction of teeth results in loss of periodontal receptors which are essential for oral-sensory functions. Osseointegrated implants have also shown to perceive signals, and this phenomenon is termed as osseoperception. The aim of this study was to determine the active tactile perception of osseointegrated dental implants supporting a fixed prosthesis in comparison to natural teeth. Materials and Methods: A total of 20 participants were included in this split-mouth cross-sectional study. The participants were divided into two groups (n = 10) as follows – Group I: participants with a restored osseointegrated dental implant in occlusion with a healthy natural tooth and Group II: participants with restored osseointegrated dental implants in occlusion. The implant-supported fixed prostheses were considered test sites whereas the natural teeth were considered control sites. Articulating papers were placed interocclusally in a predetermined random order of 50 insertions at test and control sites, respectively. The participants’ ability to perceive the presence/absence of the test papers was recorded for every insertion. Results: The minimum thickness perceived at test site was 2X that of control site in Group I whereas it was 4X the control site in Group II and the difference was statistically significant (P < 0.05). Conclusion: The results of the present study suggest that the active tactile perception of implants opposing natural teeth was nearing the natural teeth values whereas the active tactile perception of implants in opposing arches was significantly lesser.