Background: Human immunodeficiency virus (HIV) infection is an important public health globally. An important concomitant medical disorder among HIV-infected patients is tuberculosis. The management of concurrent infection between HIV and tuberculosis is usually difficult. The concomitant use of antituberculosis drug and antiretroviral drug is required. The important clinical pharmacological concern is on the possible drug–drug interaction. Methods: Here, the authors performed an informatics study. The standard drug–drug interaction analysis was done. The possible interaction between antituberculosis drug and antiretroviral drug is accessed, and the degree of severity is also predicted using a standard bioinformatics tool, drug interaction checker. Results: Several possible drug–drug interactions can be predicted through the bioinformatics analysis tool. The different degrees of interactions can be observed for different combinations between antituberculosis drug and antiretroviral drug. Conclusion: The drug–drug interaction between antituberculosis drug and antiretroviral drug is possible and can occur at various degrees. The monitoring of the concomitant use of drugs in each specific HIV-infected individual is necessary.