Cytogenetic analysis was made in female roe deer, Capreolus capreolus (Linnaeus, 1758), belonging to members of heterosexual triplets. Lymphocytic chimerism was not discovered, the cariotype being defined as 70,XX. The result obtained justifies the assumption that in cases of twin or multiple pregnancy of unlike sexes in the roe deer there is no development of infertility in the females etiologically similar to the freemartinism syndrome in domestic cattle.