Summary Human subjects were immunized against V. cholerae by the injection of vaccines which were prepared by different methods. The method of preparation did not seem to have any appreciable effect on the antibody response following the injections. Intracutaneous vaccination appeared to be more effective than subcutaneous injections. It was, however, followed in some instances by abscess formation, which although insignificant in extent and not accompanied by discomfort, should at present be considered as contraindicating its general application.