Estimation of Antibodies Specific for Dextran

descriptionPublicationkeyboard_double_arrow_right Article 01 Sep 1978 English Publisher:Oxford University Press (OUP)Journal:The Journal of Immunology, volume 121, pages 962-965 (issn: 0022-1767, eissn: 1550-6606,

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Authors: Matsuuchi, L; Morrison, S L;

doi: 10.4049/jimmunol.121.3.962

pmid: 99480

Estimation of Antibodies Specific for Dextran

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Abstract

Abstract Methods are described for the isolation and characterization of picogram quantities of anti-dextran antibodies. 14C-dextrans produced by using the dextransucrases of Leuconostoc mesenteroides strains B1355 and B512 were used in a radioimmunoassay. The specificity of this assay was verified by using cell cytoplasmic lysates from mouse plasmacytomas, J558 (anti-α 1→3 dextran) and W3129 (anti-α 1→6 dextran). Dextran produced by strain B1355 and insolubilized with epichlorohydrin was used as an immunoabsorbent.

Related Organizations

Jackson Laboratory
United States
King’s University
United States

Keywords

Immunodiffusion, Mice, Inbred BALB C, Radioimmunoassay, Dextrans, Binding, Competitive, Transplantable Tumors:, Antibodies, Antigen-Antibody Reactions, Mice, Types of Tumors:, Strains:, Antibody Specificity, Bacteria:, Animals, Neoplasm:, Serology:, Leuconostoc

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