Introduction: Exercise can reduce blood pressure (BP) levels influencing the Ankle Brachial Index (ABI) and can intervene on the development of cardiovascular diseases. Objective: To analyze the acute effects of an aerobic gymnastic session on ABI and BP levels in physically active hypertensive women. Material and Methods: This was a quasi-experimental study, in which six hypertensive women (55±3 years, 49.6±4.7 kg, 1.54±0.1 m, 23.4±1.5 kg/m2) underwent a single aerobic gymnastic session, including low-impact movements, moving, walking, going up/down a 15-cm step, jump and high-impact (running) propulsion, with three minutes for each sort of movement. The whole session lasted 60 minutes with intensity of 70-80% of the maximum heart rate. The ABI and systolic (SBP) and diastolic (DBP) blood pressure levels were measured at rest and post-exercise, using a portable vascular doppler and a sphygmomanometer, whose median values were compared using the Wilcoxon test (p 0.05). Conclusion: A single gymnastics session in hypertensive and physically active women does not change the ABI, SBP and DBP levels. Thus, this group of practitioners is not at risk for obstructive arterial disease of the lower limbs when performing such aerobic activity. DESCRIPTORS Ankle Brachial Index. Women's Health. Hypertension.