AbstractNanoparticles with diameters from 1–100 nm are used in large quantities for very different applications. A precise determination of the important physical quantities (which are responsible for the application properties) such as diameter, particle size distribution, and surface is therefore very essential. The most common methods for the determination of these quantities are analytical ultracentrifugation (AUC) and dynamic light scattering (DLS). The paper demonstrates the techniques of getting a precise characterization of nanoparticles and suggests a new method for the correction of the sedimentation coefficient and its distribution with respect to the diffusion coefficient.