The aim of this study was to evaluate the effect of the combination of carbohydrases and phytase in diets of laying hens. A total of 300 Isa Brown hens at 26 weeks were submitted to the following treatments: Positive Control: without enzyme and nutrient recovery, T2: enrichment by 1.5 and 6% AME (kcal kg-1) of corn and soybean meal, respectively, and reduction in nutrient levels as the nutrient matrix for the phytase; T3: EMA enrichment of corn and soybean meal (T2 identification) and reduced levels of calcium and phosphorus as the phytase nutrient matrix; T4: T2 supplemented with 100 g ton.-1 of carbohydrases and 30 g ton.-1 of phytase; T5: T3 supplemented with 100 g ton.-1 of carbohydrases and 30 g ton.-1 of phytase. We used a randomized design, six replications and split plot in time (6 periods of 21 days each). No significant effects of treatments were observed on production, consumption, egg weight and egg quality. The enrichment of nutrients preconized by the use of enzymes were technically efficient to maintain the performance and egg quality of laying hens in the evaluated conditions, requiring further research with greater reduction in energy levels to evaluate the effect of enzymes on the availability of energy.