Re-investigation of the chloroform extract of the aerial part of Tephrosia apollinea afforded seven new 8-prenylated flavonoids namely tephroapollin A-G (1-7). The structures were established by spectroscopic methods, including HR-CI-MS, 1 H, 13 C, DEPT, HMQC, HMBC and ROESY experiments. The relative configuration analysis of stereocenters for 5-7 was carried out on the basis of the reported J-based method. General toxicity of the isolated compounds was determined by brine shrimp lethality bioassay.