Two new acylated peonidin glycosides and three known acylated or non-acylated peonidin glycosides were identified in the flowers of 17 commercial pink petunias: peonidin 3-O-[6-O-(4-O-(4-O-(6-O-(trans-caffeoyl)-β-D-glucopyranosyl)-trans-p-coumaroyl)-α-L-rhamnopyranosyl)-β-D-glucopyranoside]-5-O-[β-D-glucopyranoside], peonidin 3-O-[6-O-(4-O-(4-O-(β-D-glucopyranosyl)-trans-p-coumaroyl)-α-L-rhamnopyranosyl)-β-D-glucopyranoside]-5-O-[β-D-glucopyranoside], peonidin 3-p-coumaroylrutinoside-5-glucoside, peonidin 3-caffeoylrutinoside-5-glucoside, and peonidin 3-rutinoside-5-glucoside.