This paper presents a methodology for localizing theoretical interchange nodes used for interchange between suburban and urban public transport in thirteen regional capitals in the Czech Republic. This methodology is based on the use of current timetables and simulation of more than 2,500 public transport connections between selected surrounding municipalities with significant commuter flows and randomly selected urban public transport stops within the regional capitals. The second part of the paper focuses on particular interchange nodes. Regional capitals are divided into groups according to the level of centrality of interchange nodes and the prevailing orientation of the transport mode. The article also analyzes the time stability of localized interchange nodes.