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Beneficial Microbes
Article . 2013 . Peer-reviewed
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Article . 2014 . Peer-reviewed
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Mobilome and genetic modification of bifidobacteria

Authors: Guglielmetti, S; Mayo, B; Alvarez-Martín, P;

Mobilome and genetic modification of bifidobacteria

Abstract

Until recently, proper development of molecular studies in Bifidobacterium species has been hampered by growth difficulties, because of their exigent nutritive requirements, oxygen sensitivity and lack of efficient genetic tools. These studies, however, are critical to uncover the cross-talk between bifidobacteria and their hosts’ cells and to prove unequivocally the supposed beneficial effects provided through the endogenous bifidobacterial populations or after ingestion as probiotics. The genome sequencing projects of different bifidobacterial strains have provided a wealth of genetic data that will be of much help in deciphering the molecular basis of the physiological properties of bifidobacteria. To this end, the purposeful development of stable cloning and expression vectors based on robust replicons - either from temperate phages or resident plasmids - is still needed. This review addresses the current knowledge on the mobile genetic elements of bifidobacteria (prophages, plasmids and transposons) and summarises the different types of vectors already available, together with the transformation procedures for introducing DNA into the cells. It also covers recent molecular studies performed with such vectors and incipient results on the genetic modification of these organisms, establishing the basis that would allow the use of bifidobacteria for future biotechnological applications.

Countries
Italy, Spain, Italy
Keywords

Genetics, Microbial, Genetic Vectors, Gene Transfer Techniques, Plasmid, Interspersed Repetitive Sequences, Bifidobacterium; bifidobacteria; plasmid; vector; genetic engineering, Bifidobacteria, Genetic engineering, Humans, Vector, Bifidobacterium, Transformation, Bacterial, Genetic Engineering, Molecular Biology, Bifidobacteria; Bifidobacterium; Genetic engineering.; Plasmid; Vector;, Plasmids

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download
citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
views
OpenAIRE UsageCountsViews provided by UsageCounts
downloads
OpenAIRE UsageCountsDownloads provided by UsageCounts
19
Top 10%
Average
Top 10%
39
24
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bronze