Counterpoising the disadvantages of low-level injec- tion efficiency and survival rates of the cell microinjection for aquatic animal used widely today,the cell automatic injection technique used in aquatic animal gene transfer is researched. The methods for transporting,holding and injecting cells automatically are presented,the cell automatic injection system is developed.A series of experiments with the fish germ cell automatic injection are performed.It is suggested by the experiments that the methods are proposed to transport germ cells,to hold germ cells and to inject germ cells automatically. The injected cells and the not injected ones would be separated and counted with the methods.The computer control process is realized.The research provided a great efficiency breeding method for cross-compatibility and improvement among species of aquatic animal.