Although there have been many recent taxonomic revisions and large-scale faunistic surveys focusing on spiders from Jiangxi Province, many taxa still remain unknown, such as the Anyphaenidae spiders. Therefore, none of the anyphaenid species has been recorded from this Province. Anyphaenidae spiders were collected from Jinggangshan National Nature Reserve, Jiangxi Province, China, during the past ten years. A new tube spider species, Anyphaena leidashi Yao & Liu, sp. nov. is diagnosed, described and illustrated. Moreover, three species including A. mogan Song & Chen, 1987, A. wuyi Zhang, Zhu & Song, 2005 and Rathalos xiushanensis (Song & Zhu, 1991) are recorded from Jiangxi Province for the first time. Distributions records are given for all investigated species.