HPV-16 E6 upregulation of DNMT1 through repression of tumor suppressor p53
Copyright policy )HPV-16 E6 upregulation of DNMT1 through repression of tumor suppressor p53
The HPV-16 early proteins E6 and E7 are considered to function as oncoproteins in cervical cancer. DNA methyltransferase 1 (DNMT1) is one of the enzymes involved in epigenetic silencing of tumor suppressor genes. In the present study, the functional role and regulation of DNMT1 in HPV-16 E6 associated cervical cancer development were examined. Knockdown of E6 in HPV-16 positive human cervical carcinoma SiHa and CaSki cells led to the increase in p53, repression of DNMT1 protein and promoter activity. Moreover, p53 knockdown increased the DNMT1 protein as well as promoter activity, indicating that p53 may mediate E6 upregulation of DNMT1. In addition, E6 knockdown induced growth retardation in SiHa cells, and the effect was partially reverted by DNMT1 overexpression. The results suggest that HPV-16 E6 may act through p53/ DNMT1 to regulate the development of cervical cancer.
- Chinese University of Hong Kong China (People's Republic of)
- The Chinese University of Hong kong Hong Kong
DNA (Cytosine-5-)-Methyltransferase 1, Human papillomavirus 16, Carcinoma, Papillomavirus Infections, Down-Regulation, Uterine Cervical Neoplasms, Oncogene Proteins, Viral, Up-Regulation, Gene Expression Regulation, Neoplastic, Repressor Proteins, Cell Line, Tumor, Gene Knockdown Techniques, Humans, Female, DNA (Cytosine-5-)-Methyltransferases, RNA, Small Interfering, Tumor Suppressor Protein p53, Cell Proliferation
DNA (Cytosine-5-)-Methyltransferase 1, Human papillomavirus 16, Carcinoma, Papillomavirus Infections, Down-Regulation, Uterine Cervical Neoplasms, Oncogene Proteins, Viral, Up-Regulation, Gene Expression Regulation, Neoplastic, Repressor Proteins, Cell Line, Tumor, Gene Knockdown Techniques, Humans, Female, DNA (Cytosine-5-)-Methyltransferases, RNA, Small Interfering, Tumor Suppressor Protein p53, Cell Proliferation
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