Using nuclear suspensions, the DNA-content of 7 bone-associated, paraffin-embedded liposarcomas and 3 lipomas were examined by means of flow cytometry (FCM) and was compared with the results of image cytophotometric measurements (ICM). Using ICM, 6 of 7 liposarcomas showed an aneuploid DNA content (86%), whereas FCM showed aneuploidy in 3 cases (43%). None of the lipomas had an aneuploid DNA-content using either method. The correspondance of the two methods was 70%. Because aneuploidy is of prognostic importance, ICM could be the method to determine the DNA content of liposarcoma.